Invitrogen™ NT-4/NTF4 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15415093	Invitrogen™ EHNTF4	96 Tests
17145529	Invitrogen™ EHNTF4X5	5 x 96 Tests

Description

The Human NeuroTrophic Factor 4 (NT-4) (Hu NTF4) ELISA quantitates Hu NTF4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NTF4. Principle of the method The Human NTF4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.

Specifications

• Accession Number: P34130
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GLC10,GLC1O,NT-4,NT-4/5,NT-5,NT4,NT5,NTF5
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 2 pg/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4909
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Apoptosis
• Synonym: GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5
• Test Time: 1 hr. 20 min.