Invitrogen™ IFN gamma Porcine ELISA Kit

Quantifies natural and recombinant swine IFN γ in serum, buffered solution, or cell culture medium

Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Product Code	Brand	Quantity
10029452	Invitrogen™ KSC4021	96 Tests
10282673	Invitrogen™ KSC4022	2 x 96 Tests

Description

The Porcine Interferon gamma (Porcine IFN-γ) ELISA quantitates Porcine IFN-gamma in serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porcine IFNγ. Principle of the method The Porcine IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN γ is a lymphokine produced by activated T and NK cells.

Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Wet Ice

Specifications

• Accession Number: P17803
• Assay Sensitivity: <2 pg/mL
• Product Type: ELISA Kit
• Target Species: Porcine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFNG
• Interassay CV: 7.8%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: IFN-γ
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Target: Porcine
• Total Assay Time: 3 hr.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 396991
• Incubation Time: 3 hr.
• Intraassay CV: 4.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.