Invitrogen™ c-Met (Soluble) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KHO2031

Product Code. 10402355

Includes: c-Met Antibody-Coated 96-Well Plate; c-Met (soluble) Standard; Standard Diluent Buffer; c-Met (soluble) Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests

Description

The Human c-Met (Soluble) ELISA quantitates Hu c-Met in human serum, plasma and cell culture supernatant. The assay will exclusively recognize both natural and recombinant Hu c-Met. Principle of the method The Human c-Met solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor receptor (HGF R), a product of the proto-oncogene c-Met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. The c-Met heterodimer is composed of an alpha chain that is disulfide-linked to a beta chain. Each alpha and beta subunit heterodimer contain 1152 amino acid residues with a calculated molecular mass of approximately 129 kDa. The alpha chain is exposed to the cell surface and the beta chain spans the plasma membrane. c-Met is synthesized as a single-chain precursor which undergoes cotranslational glycosylation and proteolytic cleavage producing the heterodimeric mature form. Human and mouse HGF receptors share 89% amino acid identity. HGF is the ligand for the HGF receptor.

Specifications

• Accession Number: P08581
• Assay Sensitivity: <0.5 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AUTS9,DFNB97,HGFR,RCCP2,c-Met
• Interassay CV: 8.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: c-Met (soluble)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 0.78-50 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 4233
• Incubation Time: 4 hr.
• Intraassay CV: 6.1%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 5 μL; Serum, 5 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 4 hr.

For Research Use Only. Not for use in diagnostic procedures.