MP Biomedicals™ Protease (S. Aureus) V.8

Protease (S. Aureus) V.8, also called endoproteinase Glu-C, Glu-C protease, staphylococcal serine proteinase. Widely used for protein sequencing, this enzyme specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids.

Brand:  MP Biomedicals™ 08399001

Product Code. 11493616

Description

• Protease V.8 specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids
• In the presence of ammonium, the specificity is limited to glutamic sites
• Used in a study to investigate immobilized residue-specific endoproteinases for protein sequencing
• Protease V8 was also used in a study to assess enzymatic cleaning of inorganic ultrafiltration membranes used for whey protein fractionation
• Provides:
  • Robust activity, greater than 915 units/mg protein
  • Specific cleavage for glutamic acid in ammonium buffer
  • Selective digestion at both glutamic and aspartic acid residues in phosphate buffer at pH 7.8
  • Cleavage activity remains in the presence of many denaturing agent, such as SDS, urea and guanidine-HCl
  • Lyophilized format for longer stability and easier storage

Specifications

• For Use With (Application): Used in a study to investigate immobilized residue-specific endoproteinases for Protein sequencing
• Sample Type: Staphylococcus aureus
• Form: Lyophilized
• Product Type: Protease

• pH Range: 4 and 7.8
• Content And Storage: 2°C to 8°C
• Quantity: 5 mg

Safety and Handling

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P321-Specific treatment (see on this label).
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.