Invitrogen™ MCPT-1 (mMCP-1) Mouse Uncoated ELISA Kit with Plates

Optimized for the accurate and precise measurement of mouse MCPT-1 in serum, and tissue culture supernatant samples. MCPT-1 (Mast Cell Protease-1) is a β-chymase, a type of serine protease stored and secreted in a tissue-specific manner by mucosal mast cells.

Brand:  Invitrogen™ 88-7503-22

Product Code. 15387697

Includes:

• Capture Antibody: Pre-titrated, purified antibody
• Detection Antibody. Pre-titrated, biotin-conjugated antibody
• Standard. Recombinant protein for generating standard curve and calibrating samples
• Coating Buffer. 10X PBS ELISA Coating Buffer
• Assay Diluent. 5X Concentrated
• Detection Enzyme. Pre-titrated Avidin-HRP
• Substrate Solution. Tetramethylbenzidine (TMB) Solution
• Certificate of Analysis. Lot-specific instructions for the dilution of antibodies and standards

Description

The Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCPT-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and is detectable in the sera of normal mice, parasites in the gut cause systemic levels to increase dramatically within two days and peak at two weeks following infection. It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed response upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, although it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving the proteins at tight junctions between cells.

Specifications

• Accession Number: P11034
• Assay Sensitivity: 120 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSIF,GVHDS,IL-10A,TGIF,IL10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

• Assay Range: 120-15,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 17224
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96-Well Plates: Corning Costar flat-bottom plated (included with catalog numbers ending in -22 and -86)
• Quantity: 2 x 96 Tests
• Sample Volume: Serum, 100 μL; Supernatant, 100 μL
• Synonym: Mouse Mast Cell Protease-1
• Test Time: 1 hr. 45 min.

For Research Use Only