Invitrogen™ Zymosan A S. cerevisiae BioParticles™, fluorescein conjugate

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

Brand:  Invitrogen™ Z2841

Product Code. 11570896

Description

• Fluorescence of fluorescein-labeled BioParticles conjugates, partially quenched in acidic environments, fluorescence of the Alexa Fluor, BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10
• Label (Ex/Em): Fluorescein (approx. 494/518nm)
• Particle: Zymosan (S. cerevisiae)
• Provided as lyophilized powders
• Approximately 3 x 10⁸ E. coli or S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
• Conjugates can be reconstituted in the buffer of choice for use in phagocytosis assays
• Fluorescence of BioParticles conjugates that are bound to surface of cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• Addition to cellular applications, fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• For Use With (Equipment): Fluorescence Microscope
• Dye Type: FITC (Fluorescein)
• pH: 3 to 10
• Quantity: 10 mg
• Species: S. cerevisiae

• Detection Method: Fluorescence
• Product Type: Bioparticle Conjugate
• Form: Solid
• Product Line: BioParticles™
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.