Invitrogen™ Transferrin From Human Serum, Tetramethylrhodamine Conjugate

Labeled LDL complexes useful in studying the delivery of up to two Fe³⁺ atoms via receptor-mediated endocytosis

Brand:  Invitrogen™ T2872

Product Code. 11580766

Description

Transferrin is monomeric serum glycoprotein (approx. 80,000 daltons) that binds to a specific receptor on surface of vertebrate cells and delivers up to two Fe³⁺ atoms via receptor-mediated endocytosis.

• Once iron-carrying transferrin proteins are inside endosomes, acidic environment favors dissociation of iron from transferrin-receptor complex
• Following release of iron, apotransferrin is recycled to plasma membrane, where it is released from its receptor to scavenge more iron
• Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish lysosomally directed and recycling endosomal pathways
• Experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy
• Label (Ex/Em): Alexa Fluor 594 (590/617)
• Amount: 15mg solid (contains 5mg of transferrin conjugate)
• Applications include: Image transferrin receptor dynamics using FRET; Observe receptor trafficking in live cells by confocal laser-scanning microscopy; Investigate events occurring during endosomal acidification; Measure transferrin receptor binding affinity in mammals and parasites

Analysis of Bacteria, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, LPS Binding, Microbiological Analysis

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Product Type: Transferrin
• Form: Solid
• Quantity: 5 mg
• Excitation/Emission: 555/580

• Detection Method: Fluorescence
• Dye Type: Classic Dyes
• pH: 7.2
• Shipping Condition: Room Temperature
• Protein Family: Transferrin

For Research Use Only. Not for use in diagnostic procedures.