Invitrogen™ DDAO Phosphate (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) Phosphate, Diammonium Salt)

Easily distinguish substrate and hydrolysis product

Brand:  Invitrogen™ D6487

Product Code. 11530346

Description

The phosphatase substrate DDAO phosphate [9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate, diammonium salt] yields a hydrolysis product that can be excited with the 633nm laser (excitation/emission maxima approx. 645/660). Although the substrate itself is fluorescent (excitation/emission maxima approx. 460/610nm), the difference between the substrate excitation maximum and that of its hydrolysis product is over 200nm, allowing the two species to be easily distinguished.

Cell Analysis, Drug Discovery and Development, Enzyme and Protein Activity Assays, Labeling Chemistry, Labeling QC and Calibration, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Form: Powder
• Substrate: Phosphatase Substrate
• Target Enzyme: Phosphatases
• For Use With (Equipment): Fluorometer, Microplate Reader
• Shipping Condition: Room Temperature

• Detection Method: Fluorescence
• Quantity: 5 mg
• Substrate Properties: Chemical Substrate
• Excitation/Emission: 646⁄659
• Label or Dye: DDAO

For Research Use Only. Not for use in diagnostic procedures.