missing translation for 'onlineSavingsMsg'
Learn More

BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 647, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen B35133

Product Code. 10335988

  • £406.00 / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.
 
This item is not returnable. View return policy

Description

Description

Cell proliferation can be measured with BrdU following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody. Although this method is rapidly being replaced with the recently developed click chemistry-based Click-iTTM EdU assay, an advantage of the antibody clone MoBU-1 is that it does not cross-react with the thymidine analog EdU (5-ethynyl-2'-deoxyuridine), thus making it valuable for use in dual-pulse applications. Traditionally, the dual-pulse method, which is used for studying biological events such as neurogenesis and cell differentiation or evaluating anti-cancer drug efficacy, employs BrdU immunocytochemistry with iododeoxyuridine (IdU) or chlorodeoxyuridine (CldU), using multiple antibodies for detection. However, using sequential pulses of the thymidine analogs EdU and BrdU greatly simplifies dual-pulse labeling, and allows a straightforward and reliable method of distinguishing BrdU- and EdU-labeled cells. In addition to no cross-reactivity with EdU, the MoBU-1 clone has a high specificity for BrdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa FluorTM conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The approximate fluorescence excitation/emission maxima for Alexa FluorTM647 conjugate are 650/665 or 670 in nm.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Order Info

Shipping Condition: Wet ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

BrdU
Monoclonal
0.2 mg/mL
PBS with 5mM sodium azide; pH 7.2
Mouse
Affinity chromatography
RUO
Chemical
Antibody
IgG
Immunohistochemistry
MoBU-1
Alexa Fluor 647
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
BrdU
350 μL
Primary
4° C
Liquid
Chemical
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.