Invitrogen™ MFG-E8 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMMFGE8X5

Product Code. 17175759

Description

The Mouse MFG-E8 ELISA quantitates Ms MFG-E8 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MFG-E8. Principle of the method The Mouse MFG-E8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human milk fat globule membrane protein (HMFG1) is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG1 is considered as a differentiation marker. It is useful as specific breast epithelial marker in normal and neoplastic mammary development.

Specifications

• Accession Number: P21956
• Assay Sensitivity: 1 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 17304
• Interassay CV: <12%
• Kit Contents: Biotinylated Detection Antibody, Adhesive Plate Covers, Stop Solution, Standard, Assay Diluent Concentrate, Wash Buffer, Pre-coated 96 well plates, Chromogen, SAV-HRP
• Quantity: 5 x 96 Tests
• Research Discipline: Angiogenesis
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.823 pg/mL to 600 pg/mL
• Calibration Range: 1 pg/mL to 600 pg/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA kit shows no cross-reactivity with the folloing cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Mfge8
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 20 μ;L; Serum, 20 μ;L; Supernatant, 50 μ;L
• Synonym: AA408458, AI325141, MFG-E8, MP47, Mfgm, P47, SED1, lactadherin
• Test Time: 1 hr. 20 min.