Molecular Probes™ LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit, for flow cytometry

The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types.

Brand:  Molecular Probes™ L34856

Product Code. 10503673

Description

The LIVE/DEAD™ BacLight™ Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of microspheres for accurate sample volume measurements.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Specifications

• Content And Storage: Contains 1 vial SYTO™ 9 nucleic acid stain (200 μL, 3.34 mM in DMSO), 1 vial of propidium iodide (200 μL, 20 mM in DMSO), and 1 vial of a microsphere standard (1.0 mL of 6.0 μm diameter microspheres).
  
  Store in refrigerator (2–8°C) and protect from light.
• Format: Tube
• Label or Dye: SYTO™ 9, propidium iodide
• Quantity: 100 reactions
• Wavelength: SYTO™ 9: 485/498, PI: 535/617

• Detection Method: Fluorescence
• Type: Bacterial Viability and Counting Kit
• Product Line: BacLight™, LIVE/DEAD™
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.