Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation

The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.

Brand:  Molecular Probes™ L34957

Product Code. 10321873

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a violet laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Low compensation

• The LIVE/DEAD™ Fixable Aqua Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser
• The aqua-fluorescent reactive dye has an excitation maximum of ∼375 nm, but it is excited well with the 405 nm violet laser
• It has an emission maxima of ∼512 nm, so it can be collected in the second channel on most violet laser flow cytometers

Specifications

• Cell Permeability: Impermeant
• Color: Aqua
• Description: LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
• For Use With (Application): Viability Assay
• Product Type: Stain
• Emission: 526 nm
• Form: Solid
• Product Line: LIVE/DEAD™
• Shipping Condition: Room Temperature

• Cell Type: Eukaryotic Cells
• Content And Storage: Contains 5 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.
  
  Store at -20°C.
  
• Detection Method: Fluorescence
• For Use With (Equipment): Flow Cytometer
• Dye Type: LIVE/DEAD™ Fixable Aqua Dead Cell Stain
• Excitation Wavelength Range: 375 nm
• Format: Tube(s)
• Quantity: 200 Assays
• Solubility: DMSO (Dimethylsulfoxide)

For Research Use Only. Not for use in diagnostic procedures.