Invitrogen™ KC/CXCL1 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMCXCL1X5

Product Code. 17105719

Description

The Mouse KC/CXCL1 ELISA quantitates Ms KC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms KC. Principle of the method The Mouse KC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Specifications

• Accession Number: P12850
• Assay Sensitivity: 1 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 14825
• Interassay CV: <12%
• Kit Contents: Assay Diluent Concentrate, Chromogen, Standard, Stop Solution, Adhesive Plate Covers, Wash Buffer, Pre-coated 96 well plates, Biotinylated Detection Antibody, SAV-HRP
• Quantity: 5 x 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.614 pg/mL to 150 pg/mL
• Calibration Range: 1 pg/mL to 150 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Fsp,Gro1,KC,Mgsa,N51,Scyb1,gro
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 33 μ;L; Serum, 33 μ;L; Supernatant, 100 μ;L
• Synonym: Fsp, Gro1, KC, Mgsa, N51, Scyb1, gro
• Test Time: 1 hr. 20 min.