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Invitrogen™ PureLink™ Genomic DNA Mini Kit

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

Brand:  Invitrogen™ K182002

384.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 1.47000kg


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Product Code. 10458843

  • £576.00 / Each
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:

Kit flexibility—one kit works with a variety of sample types and sizes
Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
Improved design—optimized spin-column design and buffer formulation for better yield and purity

Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine.

Specifications

Specifications

• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature
• 45 mL PureLink Genomic Digestion Buffer; room temperature
• 50 mL PureLinkGenomic Wash Buffer 1; room temperature
• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature
• 50 mL PureLink Genomic Elution Buffer; room temperature
• 5 mL RNase A (20 mg/mL); room temperature
• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage
• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature
• 5 x 100 PureLink Collection Tubes; room temperature

Mini
25 to 200 μL
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
250 Preps
Up to 15 min. after cell lysis
Bacteria: ≤109 cells
Cells: ≤106
Tissue: ≤25 mg
Mouse Tail: 1 cm
Rat Tail: 0.5 cm
Whole Blood: ≤1 mL
Yeast: ≤107 cells
FFPE: ≤8 sections of 5-15 μM thickness
Oragene™ Preserved Saliva: ≤4 mL
Blood Spots: ≤5 punches
Silica Spin Column
Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
gDNA
Not High-throughput Compatible (Manual)
Room Temperature
≤40 μg
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • PURELINK GENOMIC DNA KIT (250)

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Guanidine hydrochloride, Proteinase, tritirachium album serine

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.