Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

Brand:  Invitrogen™ AM8439

Product Code. 10526735

Description

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

Order Info

Shipping Conditions: Dry Ice

Specifications

• Content And Storage: 5-(3-Aminoallyl)-dUTP, 50 mM (50 μL)
  
  Store at –70°C.
• Label or Dye: Aminoallyl
• Product Type: Nucleotides
• Quantity: 50 μL
• For Use With (Application): Nucleic Acid Labeling

• Form: Liquid
• Product Line: Ambion™
• Purification Method: HPLC
• Shipping Condition: Dry Ice
• Concentration: 50 mM

For Research Use Only. Not for use in diagnostic procedures.