Invitrogen™ Osteocalcin Human Instant ELISA™ Kit

Instant ELISA Kit

Brand:  Invitrogen™ BMS2020INST

Product Code. 15571507

Includes: Aluminum pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to hOST and HRP-Conjugate (anti human Osteocalcin monoclonal (murine) antibody) and Sample Diluent, lyophilized
Aluminum pouch(es) with a human Osteocalcin Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers

Description

The Human Osteocalcin ELISA quantitates Hu Osteocalcin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Osteocalcin. Principle of the method The Human Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Specifications

• Accession Number: P02818
• Assay Sensitivity: 0.2 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BGP,OC,OCN
• Intraassay CV: 8.3%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 1.2-75 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 632
• Interassay CV: 8.1%
• Kit Contents: Pre-coated 96 well Microplate, Adhesive Plate Covers, Anti-IgG-HRP, Anti-IgG-HRP, Sample Diluent, Standard curve strips, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Lysis Buffer 10x, Lysis Buffer 10x
• Quantity: 128 Tests
• Sample Volume: Plasma, 25 μL; Serum, 25 μL; Supernatant, 25 μL
• Synonym: Gamma-carboxyglutamic acid-containing protein
• Test Time: 20 min.

For Research Use Only