Invitrogen™ IL-6 Human ELISA Kit

Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Brand:  Invitrogen™ EH2IL62

Código de producto. 10107683

Descripción

Assay IL-6 (human interleukin-6) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-6 ELISA Kits.

These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-6 (IL-6)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 10.2 to 400pg/mL

• Sensitivity: < 1pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Especificaciones

• Assay Range: 10.2 to 400pg/mL
• Description: Human IL-6 ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
• Sufficient For: 2 x 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 2 Plate Kit
• Target: Natural and recombinant human interleukin-6 (IL-6)

• Assay Sensitivity: <1pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
• Kit Contents: Biotinylated Antibody Reagent, 2 x 8mL, Anti-Human IL-6 Precoated 96-well Strip Plate, 2 ea, Adhesive Plate Covers, 12 ea, 30X Wash Buffer, 2 x 50mL, HRP Dilution Buffer, 2 x 14mL, Recombinant Human IL-6 Standard, 4 vials, Standard Diluent, 2 x 25mL, Stop Solution, 2 x 13mL, TMB Substrate Solution, 2 x 13mL
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Sample Volume: 50 μL
• Total Assay Time: 3 hr.