Invitrogen™ IL-23 Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of human IL-23 protein levels from samples including serum, plasma, and cell culture supernatants.

Brand:  Invitrogen™ 88-7237-22

Product Code. 15501087

Includes:

• Capture Antibody: Pre-titrated, purified antibody
• Detection Antibody. Pre-titrated, biotin-conjugated antibody
• Standard. Recombinant cytokine for generating standard curve and calibrating samples
• 10X Coating Buffer . Buffer for plating the Capture Antibody
• 5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
• Sample Diluent A
• Detection enzyme. Pre-titrated Avidin-HRP
• Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

The Human Interleukin 23 (IL-23) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-23. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with to two subunits, a p19 subunit unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 interacts with IL-12Rbeta1 and a beta2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated mouse and human dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. Mouse IL-23 was found not to induce significant amounts of IFN-gamma, induces strong proliferation of memory T cells (but not naive T cells), and activate mouse memory T cells to produce the proinflammatory cytokine IL-17. On the other hand, human IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-gamma, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Specifications

• Accession Number: Q9NPF7
• Assay Sensitivity: 15 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AI553453,CCP-1/C11,CCP1,Ctla-1,Ctla1,GZB
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 24 hr. 30 min.

• Assay Range: 15-2000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 51561
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Sample Diluent A: 12 mL of a 1X solution per plate, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
• Quantity: 2 x 96 Tests
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Synonym: Interleukin-23, IL23, p40, p19
• Test Time: 1 hr. 45 min.

For Research Use Only