Invitrogen™ Human IL-1R1/IL-1RA ELISA Kit

ELISA

Brand:  Invitrogen™ EHIL1R1

Product Code. 15465033

Description

The Human Interleukin 1 Receptor 1 (Hu IL-1R1/IL-1RA) ELISA quantitates Hu IL-1R1 in human serum, plasma,, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R1. Principle of the method The Human IL-1R1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The IL-1 system includes two agonists (IL-1 and IL-1), converting enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor.

Specifications

• Accession Number: P14778
• Assay Sensitivity: 6 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 3554
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Target: IL1R1
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 8.19 to 2000 pg/mL
• Calibration Range: 6 pg/mL to 2000 pg/mL
• Conjugate: HRP
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 A, IL-1 b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-g, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 A, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD121A,D2S1473,IL-1 R-alpha,IL1R,IL1RA,P80
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: CD121A, D2S1473, IL-1 R-alpha, IL1R, IL1RA, P80
• Test Time: 1 hrs 20 mins