Invitrogen™ Cytochrome C Human ELISA Kit

For the quantitative detection of human Cytochrome c.

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cytochrome c
Biotin-Conjugate anti-human Cytochrome c monoclonal antibody
Streptavidin-HRP
Human Cytochrome c Standard lyophilized, 10ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Green-Dye
Red-Dye
Adhesive Films

Product Code	Brand	Quantity
15561847	Invitrogen™ BMS263TEN	10 x 96 Tests
15541847	Invitrogen™ BMS263	96 Tests

Description

The Human Cytochrome c ELISA quantitates Hu cytochrome c in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu cytochrome c. Principle of the method The Human cytochrome c solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Accession Number: P99999
• Assay Sensitivity: 0.05 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CYC,HCS,THC4
• Intraassay CV: 6%
• Label or Dye: HRP
• Sample Volume: Cell Lysate,5 μL
• Synonym: CYC, somatic Cytochrome C, HCS
• Test Time: 1 hr. 20 min.

• Assay Range: 0.08-5.0 ng/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Application): ELISA
• Gene ID (Entrez): 54205
• Interassay CV: 4%
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

For Research Use Only