Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Product Code	Brand	Quantity	Diameter (Metric) Inner
10431065	Cytiva 17-5247-01	5 x 1 mL	7 mm
11763209	Cytiva 17-5248-01	5 mL	16 mm
11773209	Cytiva 17-5248-02	5 x 5 mL	16 mm
15167956	Cytiva 29051021	1 mL	7 mm

Description

• Prepacked with Ni Sepharose™ High Performance medium
• Negligible nickel ion leakage
• Pressure Max: 3 bar
• Column material: polypropylene
• 1 and 5mL columns
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Manual operation with syringe, pump or chromatography system

Media

• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 12

1mL columns

• Bed Dimensions: 7 x 25mm
• Flow velocity: 4mL/min.

5mL columns

• Bed Dimensions: 16 x 25mm
• Flow velocity: 5mL/min.

Specifications

• Description: HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
• Material: Polypropylene
• Support Type: 6% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)

• Flow Rate: 4 mL/min. (1 mL), 5 mL/min. (5 mL)
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C
• Type: Pre-packed Column

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains . May produce an allergic reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Ethanol, Nickel