Thermo Scientific™ HisProbe™-HRP Conjugate

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Brand:  Thermo Scientific™ 15165

Product Code. 10208163

Description

Thermo Scientific™ HisProbe-HRP is an nickel (Ni²+)-activated derivative of horseradish peroxidase (HRP). This product has been optimized for direct detection of recombinant histidine-tagged proteins and other histidine-rich proteins.

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The detection kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC).

Highlights:

• Fast – His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol

• Economical – no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies

• High specificity – HisProbe-HRP can recognize his-tags independent of adjacent tags

• Less steric hindrance – HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Specifications

• Description: Pierce HisProbe-HRP Conjugate
• Detection Method: Chemiluminescence
• Product Line: HisProbe™
• Reactivity: All species

• Product Type: HRP Conjugate
• Form: Solid
• Quantity: 2 mg
• Content And Storage: Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Safety and Handling

Product Identifier

• HisProbe-HRP Conjugate

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• Carcinogenicity Category 1A
• Germ cell mutagenicity Category 2
• Reproductive toxicity Category 1B
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1
• Specific target organ toxicity Category 1

Hazard Statement

• H302-Harmful if swallowed.
• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H341-Suspected of causing genetic defects.
• H350-May cause cancer.
• H360D-May damage the unborn child.
• H372-Causes damage to organs.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.

Supplemental information

• MIXTURE LIST-Contains : Nickel(II) chloridehexahydrate

For Research Use Only. Not for use in diagnostic procedures.