Cytiva His SpinTrap™ Spin Columns

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

Brand:  Cytiva 28-4013-53

Product Code. 11733319

Description

• Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
• One run in standard microcentrifuge takes approximatedly 10 min.
• Yields up to 750μg purified protein per column
• Allows direct purification of both clarified and unclarifed cell lysates
• Also useful for screening large numbers of small-scale bacterial lysates
• Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• Stable (long-term) in the pH range 3-12
• Average particle size: 34μm

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

• Bed Volume: 100 μL
• Support Type: 6% Highly Cross-linked Agarose
• Quantity: 1 Pack His SpinTrap Spin Columns (50 x 100 μL)
• Sample Volume: 600 μl

• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C
• Type: Spin Column Kit

Safety and Handling

Signal Word

• Warning

Hazard Category

• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : nicke, reaction massof:5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2Hisothiazol-3-one