Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Product Code	Brand	Flow Rate	Bed Volume	Diameter (Metric) Inner
11350292	Cytiva 28-9356-06	<1.3 mL/min.	120 mL	1.6cm
11330292	Cytiva 28-9356-04	<0.5 mL/min.	120 mL	1.6cm
11360292	Cytiva 28-9356-07	<0.5 mL/min.	320 mL	2.6cm

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

Column

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

HiPrep 16/60 Sephacryl S-500 HR

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107

Specifications

• Quantity: Each
• For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
• For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
• Height (Metric): 60cm
• Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
• Support Type: Allyl Dextran and N,N'-methylenebisacrylamide

• Particle Size: 25 to 75 μm
• Product Type: Sephacryl HR Pre-packed Gel Filtration Column
• Height (English): 23.62 in.
• Material: Polypropylene
• pH Range: 3 to 11
• Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids

Safety and Handling

Product Identifier

• HiPrep 16/60 Sephacryl S-500 HR

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : ethanol