Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

Brand:  Thermo Scientific™ 78427

Product Code. 11824101

Description

Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail.

• Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis
• The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate
• Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract
• The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays
• Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis
• To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis

Additional Features

• Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420)
• Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
• Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
• Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
• All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
• Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays
• Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use

Specifications

• Product Type: Phosphatase Inhibitor Cocktail
• Form: Liquid
• Reagent Type: Phosphatase Inhibitor
• Quantity: 10 mL

• Content And Storage: Upon receipt store product at 4°C. Do not freeze.
• Product Line: Halt™
• Inhibitors: Phosphatase Inhibitor

Safety and Handling

Product Identifier

• Halt Phosphatase Inhibitor Cocktail

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4

Hazard Statement

• H302-Harmful if swallowed.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : sodium fluoride, trisodium tetraoxovanadate

For Research Use Only. Not for use in diagnostic procedures.