IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity column

Strep-Tactin™ 4Flow™ high capacity gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

Brand:  IBA Lifesciences 2-1253-001

Product Code. 30117604

Description

Strep-Tactin™ 4Flow™ high capacity columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in gravity flow

Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity

4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™

Targeted elution: no tedious titration steps required and multiple regeneration cycles possible using 100 mM NaOH

Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins

Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock

Specifications

• Binding Capacity: 20 mg/mL resin
• Description: 4% agarose, crosslinked
• Form: Resin Slurry
• Protein Form: Tetrameric protein
• Type: Column
• Ligand Type: Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
• Format: Gravity flow column

• Capacity (Metric): 5 mL
• Particle Size: 50 to 150 μm
• Quantity: 5 mL
• Target: Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
• Final Product Type: Affinity chromatography equipment
• Stationary Phase: Agarose
• Column Type: Pre-packed gravity flow column