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Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Originally developed to culture human leukemic cells in suspension and as a monolayer

Brand:  Gibco™ 72400047

Code : 50

Additional Details : Weight : 0.59000kg

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Product Code. 11569726

  • £26.56 / 500mL

In stock, ready to ship: 28

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For Research Use Only. All usage must comply with product instructions.
 

Requires : Supplementation

Description

Description

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Modified as follows: with GlutaMAX™, phenol red, HEPES. RPMI 1640 medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

HEPES
Room Temperature
Gibco™, GlutaMAX™
GlutaMAX™-I
Sustainable packaging
No Sodium Pyruvate
Standard Serum Supplementation
500 ml
Liquid
Phenol Red
500mL
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Certificates

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF).