Gibco™ Recovery™ Cell Culture Freezing Medium

Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3

Brand:  Gibco™ 12648010

Product Code. 11560446

Description

For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

• Suitable for a wide variety of mammalian cells
• Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12
• Quality tested for pH, osmolality, sterility, and endotoxin
• Each lot is performance tested using CHO-K1 cells

Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR

Order Info

Shipped on dry ice.

Compliance

Manufactured at a cGMP-compliant facility registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: -5 to -20°C. Protect from light.
  Shipping conditions: Frozen
  Shelf life: 12 months from date of manufacture
• Culture Type: Mammalian Cell Culture
• Product Type: Freezing Medium
• Sterility: Sterile-filtered
• Without Additives: No Phenol Red
• Purity or Quality Grade: Research Grade
• Shipping Condition: Dry Ice

• Cell Type: Mammalian Cells
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: High Glucose, Phenol Red, DMSO (10%)
• Product Line: Gibco™, Recovery™
• Quantity: 50 mL

Safety and Handling

Product Identifier

• 3-Methyl-1-butanol

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.
• EUH066-Repeated exposure may cause skin dryness or cracking.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : 3-Methyl-1-butanol

For Research Use Only. Not for use in diagnostic procedures.