Gibco™ MEM

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Brand:  Gibco™ 11095080

Product Code. 15188319

Description

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using MEM

MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle's salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C (protect from light)
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: MEM (Minimum Essential Medium)
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Glutamine, Phenol Red
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• Sterilization Method: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.