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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Brand:  Gibco™ 31765035

Code : NEW

Additional Details : Weight : 0.59000kg

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Product Code. 15344967

  • £31.07 / 500mL

In stock, ready to ship: 21

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Requires : Supplementation

Description

Description

Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.

  • Contains zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
  • It is modified as follows: with GlutaMAX™, phenol red. Gibco F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

No HEPES
500 ml
Liquid
Phenol Red
500mL
Room Temperature
Gibco™, GlutaMAX™
GlutaMAX™-I
Sustainable packaging
Sodium Pyruvate
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For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.