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Gibco™ Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Brand:  Gibco™ 11765054

20.59 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.58000kg

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Product Code. 15172529

  • £30.89 / 500mL
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Requires : Supplementation

Description

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red

The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
Ham's F-12 Nutrient Mixture
Glutamine, Phenol Red, Sodium Pyruvate
Sustainable packaging
Gibco™
Room Temperature
CHO, COS-7, and rat prostate epithelial cells
Animal Origin-free
Liquid
Sterile-filtered
No HEPES
cGMP-compliant under the ISO 13485 standard
500 mL
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