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Gibco™ DMEM/F-12, HEPES

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12, HEPES is used in cell culture processing applications.

£20.19 - £305.00


Products 4
Product Code Brand Quantity For Use With (Application) Price Quantity & Availability  
Product Code Brand Quantity For Use With (Application) Price Quantity & Availability  
11559716
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Gibco™
11330057
10 x 500mL Mammalian cell culture
£131.00
Pack of 10
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Estimated Shipment: 23-06-2021
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11574546
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Gibco™
11330032
500mL Mammalian cell culture
£20.19
500mL
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In stock, ready to ship: 43
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12529059
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Gibco™
11330099
5L Antibody and cell culture production, Mammalian cell culture
£163.00
5 L
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In stock, ready to ship: 438
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11300882
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Gibco™
11330107
10L Antibody and cell culture production, Mammalian cell culture
£305.00
Each
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Estimated Shipment: 12-07-2021
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Description

Description

  • Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Shipping Condition: Room Temperature

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