Cytiva StrepTrap™ HP Prepacked Columns

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high.

Product Code	Brand	Quantity	Flow Rate	Content And Storage
10298754	Cytiva 28-9075-47	1 x 5mL	<20 mL/min.	4° to 8°C
11540654	Cytiva 28-9075-48	5 x 5mL	<20 mL/min.	4° to 8°C
10288754	Cytiva 28-9075-46	5 x 1mL	<4 mL/min.	2° to 8°C
15117956	Cytiva 29048653	1 x 1mL	<4 mL/min.	2° to 8°C

Description

• Prepacked with StrepTactin Sepharose High Performance
• Binding capacity Approx. 6 mg Strep II-tagged protein/ml medium
• Maximum pressure: 3 bar
• Column material: polypropylene
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Generated with 0.5M NaOH
• 1 and 5mL columns

Media

• Ligand: Streptactin
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: >7

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Product Type: StrepTactin Sepharose HP prepacked column
• Height (Metric): 25mm
• Ligand Type: StrepTactin (∼5mg/mL medium)
• pH Range: 7
• Support Type: 6% Highly Cross-linked Agarose
• For Use With (Application): Purification of Strep (II) -tagged recombinant Proteins by affinity chromato graphy

• Height (English): 0.98 in.
• Material: Polypropylene
• Max. Pressure: 3 bar
• Particle Size: 34μm
• Column Type: Ion Exchange Column

Safety and Handling

Product Identifier

• StrepTrap HP, 5 ml, 1 x 5 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol