Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Includes: Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Product Code	Brand	Quantity	Flow Rate	Bed Volume	Diameter (Metric) Inner
10320075	Cytiva 17-0716-01	5 x 1mL	4 mL/min.	1mL	0.7cm
11708478	Cytiva 17-0717-01	1 x 5mL	20 mL/min.	5mL	1.6cm

Description

Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH

• Designed for use with a syringe, peristaltic pump, or chromatography system
• Degree of substitution: 10 μmol NHS/mL medium
• Delivered in 100% isopropanol to protect the active groups
• Stable in the pH range 3 to 12, depending upon ligand
• Average particle size: 34μm

Specifications

• Particle Size: 34 μm
• Product Type: NHS-Activated HP Column
• Height (English): 0.98 in.
• Ligand Type: 10μM NHS groups/mL medium
• Max. Pressure: 42psi
• Support Type: 6% Highly Cross-linked Agarose

• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
• For Use With (Application): Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
• Height (Metric): 2.5cm
• Material: Polypropylene
• pH Range: 3 to 12
• Content And Storage: 4° to 8°C

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : propan-2-ol