Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Product Code	Brand	Quantity
11595015	Cytiva 17-5132-02	100 mL
11585015	Cytiva 17-5132-01	25 mL
11505025	Cytiva 17-5132-03	500 mL

Description

• Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
• Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
• Binding capacity and high flow properties allow scale-up
• Mild elution conditions preserve protein antigenicity and function
• Stable in all commonly used aqueous buffers
• pH stability: 3 to 12

Specifications

• Binding Capacity: >10 mg/mL
• Flow Rate: <450 cm/hr.
• Matrix: Highly cross-linked 4% agarose
• Support Type: 4% Highly Cross-linked Agarose
• Max. Operating Pressure: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Type: Chromatography Media

• Description: Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
• Kit Contents: 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 90 μm
• Ligand Type: Glutathione and 10-carbon linker arm