Perform silica bead-based DNA extraction from agarose gels and reaction mixtures with this kit, which is flexible to scale up or down both reaction and elution volumes.
Brand: Thermo Scientific™ K0513
115.90 GBP valid until 2025-12-31
Use promo code "24339" to get your promotional price.
Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie , employing solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water.
This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling reaction and elution volumes up or down. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.
Highlights
| Kit | |
| Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing | |
| Silica Bead DNA Gel Extraction Kit | |
| DNA from Enzymatic Reactions (e.g. PCR) |
| DNA, Gel Samples | |
| Not High-throughput Compatible (Manual) | |
| 100 Preps | |
| 15 to 20 min. |
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For Research Use Only. Not for use in diagnostic procedures.
