Thermo Scientific™ Silica Bead DNA Gel Extraction Kit

Perform silica bead-based DNA extraction from agarose gels and reaction mixtures with this kit, which is flexible to scale up or down both reaction and elution volumes.

Brand:  Thermo Scientific™ K0513

Product Code. 10202540

Description

Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie , employing solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water.

This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling reaction and elution volumes up or down. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.

Highlights

• Flexible—easy to scale up or down both reaction and elution volumes
• Efficient—greater than 80% DNA recovery
• Fast—purification takes only 15 to 20 minutes
• Effective—suitable for DNA fragments as short as 100 bp
• Safe—does not involve phenol extraction

Specifications

• Format: Kit
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Nucleic Acid Labeling, Sequencing
• Product Type: Silica Bead DNA Gel Extraction Kit
• Target: DNA from Enzymatic Reactions (e.g. PCR)

• Sample Type: DNA, Gel Samples
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 100 Preps
• Test Time: 15 to 20 min.

Safety and Handling

Product Identifier

• Silica Bead DNA Gel Extraction Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 1 B

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P363-Wash contaminated clothing before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Thiocyanic acid

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For Research Use Only. Not for use in diagnostic procedures.