Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)

The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).

Product Code	Brand	Quantity
10558170	Thermo Scientific™ ER1641	200 U
10764501	Thermo Scientific™ ER1642	1000 U

Description

Lambda DNA digested with SatI (Fnu4HI), 1.4% agarose, 380 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality -stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Specifications

• Concentration: 10 U/μL
• Enzyme: SatI (Fnu4HI)
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: No
• Research Category: Traditional Cloning

• Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
• Compatible Buffer: 10X Buffer G
• Optimal Reaction Temperature: 37°C
• Product Type: Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures.