Maxima Miscela master per qPCR Maxima™ SYBR™ Green 2X con soluzione ROX™

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Marca:  Maxima K0252

Codice prodotto. 11863923

Descrizione

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

• Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity - detects low copy number targets
• Wide linear range - accurate quantification across 9 orders of magnitude
• Reproducibility and convenience - ready-to-use 2X master mix

Specifica

• Content And Storage: Contains:
  • 10 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions.
  • 250 μL ROX solution, 50 μM
  • 10 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Formato: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Linea di prodotti: Maxima
• Sufficiente per: 1000 Reactions at 25 μL
• Concentrazione: 2X
• Tipo di campione: DNA, dsDNA

• Metodo di rilevamento: SYBR
• GC-Rich PCR Performance: High
• Polimerasi: Taq DNA Polymerase
• Da utilizzare con (apparecchiatura): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Type: Real Time PCR SYBR Master Mix
• Da utilizzare con (applicazione): Gene Expression
• N. di reazioni: 1000 reazioni da 25 µl
• Volume: 10 x 1.25 mL

For Research Use Only. Not for use in diagnostic procedures.