Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Brand:  Thermo Scientific™ K0262

Product Code. 12201547

Description

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

The master mix includes Maxima Hot Start Taq DNA polymerase and dNTPs in an optimized PCR buffer. ROX Solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima Hot Start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity.dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• ROX Solution provided in a separate tube
• Wide linear range—accurate quantification across 9 orders of magnitude
• Universal—can be used on most real-time PCR instruments
• Reproducibility and convenience—ready-to-use 2X master mix

Recommended for:

Real-time PCR and RT-qPCR using sequence specific probes on most real-time PCR machines.

Specifications

• Content And Storage: Contains:
  • 10 x 1.25 mL Maxima Probe qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer; sufficient for 1000 x 25 μL reactions.
  • 250 μL ROX solution, 50 μM
  • 10 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: Maxima
• Quantity: 1,000 reactions
• For Use With (Application): Gene Expression
• No. of Reactions: 1,000 Reactions
• Volume: 10 x 1.25 mL

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
• Product Type: qPCR Master Mix
• Sufficient For: 1000 Reactions at 25 μL
• Concentration: 2X
• Sample Type: DNA, dsDNA

For Research Use Only. Not for use in diagnostic procedures.