Gibco™ Essential 8™ Medium

Essential 8™ Medium

Brand:  Gibco™ A1517001

Product Code. 15190617

Description

Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8™ Medium include:

Consistent—reduced variability compared to existing feeder-free culture media

Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium

Cost effective—economical and scalable PSC culture compared to other feeder-free media

Reduced Variability
Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures
Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective
Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.

Specifications

• Content And Storage: • 500 mL basal medium (store at 2—8°C and protect from light)
  • 10 mL supplement (store at -5 to -20°C and protect from light)
• Classification: Xeno-free
• Form: Liquid
• Serum Level: Serum-free
• Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
• Quantity: 500 mL
• Species: Human

• Cell Type: Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs) 
• Culture Type: Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
• Product Type: Stem Cell Media
• With Additives: Phenol Red
• Product Line: Essential 8™
• Shipping Condition: Supplement - dry ice, Basal - ambient

Safety and Handling

Product Identifier

• 3,4-Dihydroxy-3-cyclobutene-1,2-dione

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : 3,4-Dihydroxy-3-cyclobutene-1,2-dione

For Research Use Only. Not for use in diagnostic procedures.