Invitrogen™ Eotaxin-1 (CCL11) Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMCCL11X5

Product Code. 17115689

Description

The Mouse Eotaxin-1/CCL11 ELISA quantitates Ms Eotaxin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-1. Principle of the method The Mouse Eotaxin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

AHC6054CU recognizes natural and recombinant eotaxin.

Specifications

• Accession Number: P48298
• Assay Sensitivity: 1 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 20292
• Interassay CV: <12%
• Kit Contents: Standard, Pre-coated 96 well plates, Assay Diluent Concentrate, Stop Solution, Adhesive Plate Covers, SAV-HRP, Chromogen, Wash Buffer, Biotinylated Detection Antibody
• Quantity: 5 x 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 1.02 pg/mL to 100 pg/mL
• Calibration Range: 1 pg/mL to 100 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF)
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Scya11,eotaxin
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μ;L; Serum, 10 μ;L; Supernatant, 100 μ;L
• Synonym: Scya11, eotaxin
• Test Time: 1 hr. 20 min.