IBA Lifesciences CD45RA Fab-TACS™ Magnetic Microbead Starter Kit

Label-free isolation of CD45RA+ cells from PBMCs

Brand:  IBA Lifesciences 6-8000-108

Product Code. 17217760

Description

• Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology. CD45RA Fab-TACS™ Magnetic Microbead Starter Kit allows label-free isolation of human CD45RA⁺ cells from peripheral blood mononuclear cells (PBMCs).
• CD45RA is an isoform of tyrosine phosphatase CD45 (leukocyte common antigen) that is expressed on different leukocytes including naïve and resting T cells, B cells as well as monocytes
• Low affinity, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) are incubated with Strep-Tactin™ Magnetic Microbeads to form a complex that stably binds to the cell surface. After specific labeling, target cells are bound to a magnet and non-target cells efficiently washed away.
• In a final step, the addition of biotin causes the release of the isolated cells from magnetic microbeads. The Fab-Streps self-dissociate from the cell surface due to their low affinity.
• After removing biotin by washing, the isolated cells are ready for further positive selection or depletion steps or can directly be used for further downstream applications

Specifications

• Includes: Strep-Tactin™ Magnetic Microbeads, CD45RA Fab-Strep (human), 10 x Buffer CI, Biotin stock solution
• Target Species: Human
• For Use With (Application): Label-Free Isolation of CD45RA+ Cells From PBMCs

For Research Use Only.