IBA Lifesciences CD4 Fab-TACS™ Agarose Column Starter Kit

Label-free isolation of CD4+ T cells

Brand:  IBA Lifesciences 6-3301-002

Product Code. 17267740

Description

• Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology
• CD4 is a co-receptor that associates with the T cell receptor and interacts with MHC class II molecules
• Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix.
• Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away.
• In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity
• The label-free authentic target cells are now ready for further downstream applications

Specifications

• Includes: Strep-Tactin™ TACS Agarose columns, 0.3 mL (2 x), CD4 Fab-Strep (mouse), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter
• Donor Source: Murine splenocytes
• Target Species: Mouse
• Buffer: 10 x Buffer CI

For Research Use Only.