IBA Lifesciences CD19 Fab-TACS™ Agarose Column Starter Kit

Label-free isolation of CD19+ B cells from whole blood, buffy coat or PBMCs

Brand:  IBA Lifesciences 6-3213-002

Product Code. 17247740

Description

• Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology
• CD19 Fab-TACS™ Isolation Kit allows label-free isolation of human CD19⁺ B cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD19 is an adaptor protein that is involved in signaling pathways
• Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix.
• Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away.
• In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity. The label-free authentic target cells are now ready for further downstream applications.

Specifications

• Includes: Strep-Tactin™ TACS Agarose columns, 1 mL (2 x), CD19 Fab-Strep (human), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter
• Target Species: Human

For Research Use Only.