IBA Lifesciences BioLock

BioLock is a Biotin blocking solution for lysates and culture supernatants

Product Code	Brand	Capacity (Metric)
15643608	IBA Lifesciences 2-0205-050	50 mL
15653608	IBA Lifesciences 2-0205-250	250 mL

Description

BioLock is a ready-to-use biotin blocking solution which contains the tetrameric biotin binding protein avidin. BioLock prevents biotin and biotinylated proteins from binding to Strep-Tactin™ or Strep-Tactin™XT, whereas binding of the Twin-Strep-tag™ and Strep-tag™II is not influenced.

Biotin and/or biotinylated proteins can possibly reduce the binding capacity of Strep-Tactin™ as well as Strep-Tactin™XT and are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag™ and Strep-tag™II fusion proteins from biotin-containing extracts or media are intended to be purified via Strep-Tactin™ or Strep-Tactin™XT affinity chromatography, biotin has to be masked by addition of BioLock prior to application onto the resin. 

Specifications

• Description: Activity: >70 U/ mL
• Type: Blocking Solution

For Research Use Only.