Invitrogen™ BDNF Rat ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ ERBDNFX5

Product Code. 17125909

Description

The Rat Brain-Derived Neurotrophic Factor (Rt BDNF) ELISA quantitates Rt BDNF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt BDNF. Principle of the method The Rat BDNF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Accession Number: P23363
• Assay Sensitivity: 12 pg/mL
• Description: BDNF ELISA Kit, 96 Assays, 1 Kit, Rat Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BDNF in Samples Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Protein Family: Growth Factors & Receptors
• Specificity: This ELISA pair antibody detects rat and human BDNF. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 24225
• Host Species: Rat
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
• Target: Rat
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 12.29 pg/mL to 3000 pg/mL
• Calibration Range: 12 pg/mL to 3,000 pg/mL
• Detectable Analytes: Rat and human BDNF
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Bdnf
• Interassay CV: <12%
• Kit Contents: Anti-rat BDNF precoated 96-well strip plate, lyophilized recombinant rat BDNF standard, 20 x wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2 M sulfuric acid, assay diluent (5 x concentrated bμf)
• Quantity: 5 x 96 Tests
• Research Discipline: Neurobiology
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.