ATP1A1 Monoclonal Antibody (M8-P1-A3), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA3929

Product Code. 11535812

Description

MA3-929 detects sodium/potassium ATPase alpha 1 in sheep, canine, rat, mouse, human and porcine tissues. This antibody does not detect the alpha 1 isoform in Xenopus and chicken. MA3-929 does not detect the alpha 2 or alpha 3 isoform in rat. MA3-929 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry procedures. By Western blot, this antibody detects an ∽110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract. Immunohistochemical staining of sodium/potassium ATPase alpha 1 with MA3-929 yields a pattern consistent with plasma membrane localization. The MA3-929 antigen is sheep kidney alpha 1 sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: ATP1A1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P04074, P05023, P05024, P06685, P50997, Q8VDN2
• Gene Symbols: ATP1A1
• Immunogen: Lamb kidney alpha 1 sodium/potassium ATPase.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Ovine, Porcine, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: M8-P1-A3
• Conjugate: Unconjugated
• Gene: ATP1A1
• Gene Alias: (Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; alpha subunit of sodium potassium ATPase; atp1a1; atp1a1-a; atp1a1a.4; atp1a1-b; ATP1B; Atpa-1; ATPase; ATPase Na+/K+ transporting subunit alpha 1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; BC010319; K-ATPase; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na/K ATPase alpha 1 isoform; Na/K ATPase alpha 1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ ATPase; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ -ATPase alpha 1 subunit; Na+/K+ transporting alpha 1 polypeptide; Na+K+ ATPase; Na+K+ATPase; Nkaa1b; RP4-655J12.1; sarcolemmal sodium/potassium-ATPase alpha 1; Sodium Potassium ATPase; sodium pump 1; sodium pump subunit alpha-1; sodium/potassium-ATPase alpha-subunit 1; sodium/po
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 11928, 24211, 397481, 403992, 443381, 476
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid