HSF2 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA530064

Product Code. 13454597

Description

Recommended positive controls: A549, mouse liver. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (81%), Chicken (87%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

Specifications

• Antigen: HSF2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HSF2
• Gene Alias: AI661205; heat shock factor 2; heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; Hsf2; HSTF 2; HSTF2; MGC117376; MGC156196; MGC75048
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15500, 3298
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P38533, Q03933
• Gene Symbols: HSF2
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 188 of Human HSF2
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG