Dystrophin Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA137587

Product Code. 11531823

Description

This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

Specifications

• Antigen: Dystrophin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DMD
• Gene Alias: apodystrophin-3; apodystrophin-I; BMD; CMD3B; dmd; dmd.1; dmd.1.L; DNADMD1; Dp427; Dp71; Duchenne muscular dystrophy (DMD); DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; Dystrophin; dystrophin Dp40; dystrophin Dp71 isoform; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; dystrophin, gene 1 L homeolog; dystrophin, muscular dystrophy; GS1-19O24.1; mdx; MRX85; Muscular dystrophy Duchenne and Becker types; OTTHUMP00000215592; pke; utrn; utrophin; XELAEV_18012903mg; X-linked muscular dystrophy
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13405, 1756
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Concentration: Conc. not determined
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P11531, P11532
• Gene Symbols: DMD
• Immunogen: Synthetic peptide derived from C-terminus of human dystrophin.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG