Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Product Code	Brand	Quantity
10665795	Invitrogen™ AM2546	1.25 mL
10259184	Invitrogen™ AM2548	5 x 1.25 mL

Description

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K remains active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Order Info

Shipping Condition: Dry Ice

Specifications

• Concentration: 20 mg/mL
• Content And Storage: Store at -20°C.
  Shipped at ambient temperature.
• Product Type: Proteinase K
• Form: Liquid

• Host Species: T. album
• Shipping Condition: Dry Ice
• Product Line: Ambion™

Safety and Handling

Product Identifier

• PROTEINASE K (20MG/ML) 1.25ML

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : Proteinase, tritirachiumalbum serine

For Research Use Only. Not for use in diagnostic procedures.